Thesis Statement For Romeo And Juliet Essay Example For Students

Thesis Statement For Romeo And Juliet Essay Everyday there is something that one must interpret whether it is a book, movie, or even the news. One must come to ones own conclusion about the information they are interpreting. For instance movies are mostly easier to understand then books though the plots may have slight differences. In the 1996 movie The Tragedy Of Romeo and Juliet by Baz Luhrman, there are some major differences between his version and the book Romeo and Juliet by William Shakespeare. One of the major differences is that the book takes place in the seventeen hundreds in Verona, while the movie takes place in the late twentieth century still in Verona. This difference makes the movie to appear of a rather young intellect due to the fact that the movie uses the same language as the book. It seems out of place. We will write a custom essay on Thesis Statement For Romeo And Juliet specifically for you for only $16.38 $13.9/page Order now Another major difference is the role of friar Lawrence. In the book the friar had a part of great importance yet in the movie his importance does diminish some. One more major difference is the Capulets tomb. In the book the tomb was said to be dark and dingy. Though in the movie the tomb appeared to be a beautiful almost church like cathedral the tomb had many candles around where Juliet laid. Also she does not lie in a coffin but in a beautiful bed that is encompassed by white sheets. Perhaps the most important difference between the book and the movie is the final scene. In the book the final scene is when Romeo kills Paris and then enters Capulets tomb. When in the tomb Romeo drinks the poison and dies. However in the movie Romeo does not kill Paris at all. When Romeo arrives at the tomb the police surround him. He takes a priest as a hostage so he wont be shot. When he enters the tomb he walks to Juliet. This is where the most important difference is. Juliet starts to awaken while Romeo recites his speech. They are both alive and looking at each other for a few seconds then Romeo dies. The biggest controversy is if Juliet had even utter ed the faintest noise it may have stopped Romeo from drinking the poison. When a person interprets anothers work the interpreter may loose key values and change the story completely. Therefore the interpreter must be extremely careful. As in the case of The Tragedy of Romeo and Juliet, by Baz Luhrman.

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Free Essays on Huffy Sports Free Essays on Huffy Sports Junior Diagnostic Project Huffy Sports Table of Contents Company Background Huffy Sports Info †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦ 3 Product and Problem †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦3-4 What I Would Do †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦4 Consumer Issues Perceived Risks †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦ 5 Problem Recognition †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦5-6 Consumer Search Behavior †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦ 7 Consumer Behaviors †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦7-8 Message Content †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦ 8 Suggestions and Analysis of Consumer Issues Perceived Risks †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦ 9 Problem Recogntion †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦ 9 Consumer Search Behavior †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦9-10 Consumer Behaviors †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦ 10 Message Content †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦10-11 †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦12 Diagnostic Project: Huffy Sports Huffy Sports Company of Sussex, Wisconsin is the leading manufacturer of basketball related products in the world. 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Mobile Plus Group Ltd market analysis Research Paper

Mobile Plus Group Ltd market analysis - Research Paper Example Mobile Plus Group Ltd market analysis Business people who are value conscious will be able to appreciate the savings that our dual and triple SIM cellphones can offer as it affords them an extra line without the additional cost of an extra phone. Business venturers usually contact different kinds of people from different society classes thus using all the available network services that there is. Most of the time, these networks compete each other and offer promos that are only exclusive to a Same Network basis. In this case, business people tend to switch from one SIM to the other just to avail of the promos whenever they use their cellphones to call their wide array of clients who use different Networks. That is the reason why to target business people in selling dual and/or triple SIM cellulars is a practical idea to pursue.Giving them the freedom to use at most, three different Networks at once to contact their clients without having to switch phones offers efficiency and savings from the costs of buying another cell ular phone. This will also offer a better transacting means for business users who would usually organize in just a single phone the events that they have to meet throughout their working schedules.Personal users – Our secondary market will be personal cellphone users or the general public. Personal cellphone users can be a profitable segment in the market given their sheer size. The consumer’ behavior and buying preference are influence by several factors. Typical example of how these behavior and preference is influenced by the environmental factors which are media, culture and social influences.

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Research Proposal - Essay Example It is in this way that the dual nature of the data demanded will be taken care of. However, it should be pointed that on the basis of the literature review, it is established that low bone content is associated with sedentary lifestyle. A good number of high school students have been found to suffer from a number of deformities or anomalies. The low bone mineral associated with a number of the school population has been attributed to the nature of lifestyle that these people lead. It is found that sedentary students are more prone to this condition as opposed to athletic students albeit on the basis of this study. Through the use of both quantitative and qualitative approaches of data collection, a research is conducted to confirm the veracity of the statement or deny it. On the basis of the literature review, it is found that indeed sedentary students have less bone mineral concentration when compared to the athletic students. It is also found that low bone formation or content does have an adverse effect on the formation of the body skeleton. In turn, poor skeletal formation is known to lead to overall body malfunctioning. Hoch et al. (2009) claims that a substantial number of high school athletes (78%) and a surprisingly high number of sedentary students (65%) have one of or more components of the female athlete triad. The female athlete triad is a co-morbidity condition which refers to an Eating Disorder (low calorie intake), Amenorrhoea (menstrual abnormality) and Osteoporosis (low bone density) (Shamley, 2005). The scope of this research proposal is to investigate among collegiate females if the decline of bone density is strong correlate to a sedentary life style. It is thus presumed that the more sedate an individual is the more their chances of developing the condition. Hoch et al. (2009) found that sedentary students had a bone density lower than student athletes. The assumption is

Aging out of the adoption system in Massachusetts Research Paper

Aging out of the adoption system in Massachusetts - Research Paper Example Aging out of the Adoption System in Massachusetts In 2009 approximately 423,773 children nationwide were in the foster care system of that number some were adopted, but for others that remained in the system the experiences of their adolescence are times to be quickly forgotten and put far into the past Annually, approximately 24,000 children age out of the foster system that number excludes those that run away and those reunited with family members. In most states foster care stipulations do not provide assistance once a child has his or her 18th birthday This paper will list some of the past and current child welfare policies enacted in the United States that are actively providing assistance to states to alleviate the problems that youth aging out of the foster care system are encountering. Additionally, highlighting the State of Massachusetts discussing the innovative and creative methods state administrators are developing to provide substantial assistance to children transition ing from foster care to independence (Commonwealth of Massachusetts, 2011). The final talks will provide an analysis of the effectiveness of the programs and policies, reviewing the current trends, and proposals states are offering as possible ways to make the transition from foster care to adulthood smoother and less problematic. Aging Out Emancipated or â€Å"aged out† are terms for children in the foster care system that have reached their 18th birthday and are no longer eligible for services under the foster care auspice. The information about what happens to children after aging out of the foster care system consistently recite the plight of homelessness, unemployment, criminal activity, uneducated, unplanned parenthood, and other conditions appearing chronic for youth aging out of the system (Gardner, 2008; Leigh, et al, 2007; Keller, 2007). Whereas the problems facing youth aging out of the foster care system is systemic with some areas o experiencing a set of differen t problems, each state is responsible for establishing and managing their individual state’s issues. The U.S. Department of Health and Human Resources and the Social Security Administration are the primary federal agencies providing funding to states that develop and administer programs addressing the issues pertaining to the welfare of children (U. S. Department of Health & Human Resources, 2011). In 2009 roughly 29, 471 children were emancipated, 6% or 16,323-16 year olds; 6% or 17,514-17 year olds; and 10% or 26,416 were 18 years of age (United States Department of Health and Human Services, 2011). Children in foster care typically do not have any relationships with former family members such as grandparents, uncles and aunts. Because of the multiple placements in the foster care system children infrequently establish any consistent relationship other than with social service workers, therefore the transition from care to adulthood or self-sufficiency is not only emotional ly traumatic but one in which the future looks bleak and lonely Because of the instability in the foster system and the multiple foster placements many children do not meet the minimum educational standards as a result with many failing to graduate from high school (Shin, 2009). The Chafee Education and Training Vouchers Program (ETV) provide $5,000 annually per child and enables states to provide GED certification for former foster children that did not complete high school. This program addresses the problem of foster youth not having the educational aptitude and ability to become self-sufficient and with the proper mentoring encourage youth to pursue secondary education. In 2009 through Congressional allocations of over 60 million dollars were set aside for states to use for educating foster alumni (U.S.

Overview of Cytokinins (CK)

Overview of Cytokinins (CK) FARA SHAKEEL CYTOKININS: Cytokinins (CK) are a class of plant growth substances (phytohormones) that promote cell division, or cytokinesis, in plant roots and shoots. They are involved primarily in cell growth and differentiation, but also affect apical dominance, axillary bud growth, and leaf senescence. STRUCTURE OF CYTOKININS: NATURE OF CYTOKININS: Cytokinins are compounds with a structure resembling adenine which promote cell division and have other similar functions to kinetin. Kinetin was the first cytokinin discovered and so named because of the compounds ability to promote cytokinesis (cell division). Though it is a natural compound, It is not made in plants, and is therefore usually considered a synthetic cytokinin (meaning that the hormone is synthesized somewhere other than in a plant). The most common form of naturally occurring cytokinin in plants today is called zeatin which was isolated from corn (Zea mays) Cytokinins have been found in almost all higher plants as well as mosses, fungi, bacteria, and also in tRNA of many prokaryotes and eukaryotes. Today there are more than 200 natural and synthetic cytokinins combined. Cytokinin concentrations are highest in meristematic regions and areas of continuous growth potential such as roots, young leaves, developing fruits, and seeds (Arteca, 1996; Mauseth, 1991; Raven, 1992; Salisbury and Ross, 1992). HISTORY OF CYTOKININS: In 1913, Gottlieb Haberlandt discovered that a compound found in phloem had the ability to stimulate cell division (Haberlandt, 1913). In 1941, Johannes van Overbeek discovered that the milky endosperm from coconut also had this ability. He also showed that various other plant species had compounds which stimulated cell division (van Overbeek, 1941). In 1954, Jablonski and Skoog extended the work of Haberlandt showing that vascular tissues contained compounds which promote cell division (Jablonski and Skoog, 1954). The first cytokinin was isolated from herring sperm in 1955 by Miller and his associates (Miller et al., 1955). This compound was named kinetin because of its ability to promote cytokinesis. Hall and deRopp reported that kinetin could be formed from DNA degradation products in 1955 (Hall and deRopp, 1955). The first naturally occurring cytokinin was isolated from corn in 1961 by Miller (Miller, 1961). It was later called zeatin. Almost simultaneous w ith Miller Letham published a report on zeatin as a factor inducing cell division and later described its chemical properties (Letham, 1963). It is Miller and Letham that are credited with the simultaneous discovery of zeatin. Since that time, many more naturally occurring cytokinins have been isolated and the compound is ubiquitous to all plant species in one form or another (Arteca, 1996; Salisbury and Ross, 1992). BIOSYNTHESIS AND METABOLISM: Cytokinin is generally found in higher concentrations in meristematic regions and growing tissues. They are believed to be synthesized in the roots and translocated via the xylem to shoots. Cytokinin biosynthesis happens through the biochemical modification of adenine. The process by which they are synthesized is as follows (McGaw, 1995; Salisbury and Ross, 1992): A product of the mevalonate pathway called isopentyl pyrophosphate is isomerized. This isomer can then react with adenosine monophosphate with the aid of an enzyme called isopentenyl AMP synthase. The result is isopentenyl adenosine-5-phosphate (isopentenyl AMP). This product can then be converted to isopentenyl adenosine by removal of the phosphate by a phosphatase and further converted to isopentenyl adenine by removal of the ribose group. Isopentenyl adenine can be converted to the three major forms of naturally occurring cytokinins. Other pathways or slight alterations of this one probably l ead to the other forms. Degradation of cytokinins occurs largely due to the enzyme cytokinin oxidase. This enzyme removes the side chain and releases adenine. Derivitives can also be made but the pathways are more complex and poorly understood. TRANSPORT: via xylem (transpiration stream) in peas, a signal from the leaves may signal/regulate transport of cytokinins from the roots zeatin ribosides are the main transport form; converted to the free base or glucosides in the leaves some cytokinin also moves in the phloem. Mode of action: The ratio of auxin to cytokinin plays an important role in the effect of cytokinin on plant growth. Cytokinin alone has no effect on parenchyma cells. When cultured with auxin but no cytokinin, they grow large but do not divide. When cytokinin is added, the cells expand and differentiate. When cytokinin and auxin are present in equal levels, the parenchyma cells form an undifferentiated callus. More cytokinin induces growth of shoot buds, while more auxin induces root formation. Cytokinins are involved in many plant processes, including cell division and shoot and root morphogenesis. They are known to regulate axillary bud growth and apical dominance. The direct inhibition hypothesis posits that these effects result from the cytokinin to auxin ratio. This theory states that auxin from apical buds travels down shoots to inhibit axillary bud growth. This promotes shoot growth, and restricts lateral branching. Cytokinin moves from the roots into the shoots, eventually signaling lateral bud growth. Simple experiments support this theory. When the apical bud is removed, the axillary buds are uninhibited, lateral growth increases, and plants become bushier. Applying auxin to the cut stem again inhibits lateral dominance. While cytokinin action in vascular plants is described as pleiotropic, this class of plant hormones specifically induces the transition from apical growth to growth via a three-faced apical cell in moss protonema. This bud induction can be pinpointed to differentiation of a specific single cell, and thus is a very specific effect of cytokinin. Cytokinins have been shown to slow aging of plant organs by preventing protein breakdown, activating protein synthesis, and assembling nutrients from nearby tissues. A study that regulated leaf senescence in tobacco leaves found that wild-type leaves yellowed while transgenic leaves remained mostly green. It was hypothesized that cytokinin may affect enzymes that regulate protein synthesis and degradation. CHEMISTRY: A. General adenine derivatives (amino purines) occur as: (a) the free nitrogenous base; (b) a nucleoside (base + ribose); (c) a nucleotide (base + ribose + phosphate); or (d) glycosides The free base is the active form. approximately 40 different structures known. Zeatin (Z), which was first isolated from maize (Zea mays) is the most common cytokinin. Other naturally occurring cytokinins include, dihydrozeatin (DHZ) and isopentenyladenosine (IPA). B. Synthetic cytokinins kinetin – probably byproduct of zeatin degradation there are several other substances with cytokinin activity such as benzyl adenine (benzylaminopurine; BA). C. Cytokinins and nucleic acids can occur as a modified base in tRNA, but the bases exist in the cis form, rather than the typical trans form. These modified bases that are found in all organisms from bacteria to plants to humans. The function of the tRNA cytokinins is not clear, but after hydrolysis of the tRNA the products can act as a cytokinin. The importance of the tRNA derived cytokinins in overall growth and development is not clear, either. Interestingly plants have different sets of tRNA’s with different cytokinins that participate in protein synthesis in the cytoplasm and the plastids. CYTOKININS FUNCTION: A list of some of the known physiological effects caused by cytokinins are listed below. The response will vary depending on the type of cytokinin and plant species (Davies, 1995; Mauseth, 1991; Raven, 1992; Salisbury and Ross, 1992). Stimulates cell division. Stimulates morphogenesis (shoot initiation/bud formation) in tissue culture. Stimulates the growth of lateral buds-release of apical dominance. Stimulates leaf expansion resulting from cell enlargement. May enhance stomatal opening in some species. Promotes the conversion of etioplasts into chloroplasts via stimulation of chlorophyll synthesis ROLE OF CYTOKININS IN PLANTS: Cytokinins are known from cytokinin-overproducing mutants to produce additional leaves and branches on the stem. The stems and leaves produce additional chlorophyll. Wounding often produces a new branch. Leaf senescence is delayed. Apical dominance is released. Cuttings produce adventitious roots slowly and require additional auxin to reliably root. Tumors may form at nodes. The results of applied cytokinins could include release of apical dominance as you demonstrated in kidney beans in laboratory. Cytokinins regulate the cell cycle as we learned early in the semester. Cytokinins delay senescence. You carried out this project at home with isolated wheat primary leaf tips in various solutions of plant hormones. Those treated with cytokinins should have demonstrated delayed senescence (stayed green longer). Of course, remember the auxin dose responsethere is such a thing as too much of a good thing! Cytokinins cause nutrient diversion. Cytokinin-treated leaves become sinks for nutrients such as amino acids. This is shown in a classic experiment in plant physiology CYTOKININS CONJUGATION: As you can see in the diagram below, the natural cytokinin ribosides can be attached to sugars to form glycosides that have reduced or no cytokinin activity. The plant also can produce enzymes to cleave the sugar and restore full cytokinin activity. Thus conjugation with sugars and retrieval from these bound forms is a possible pathway in plant cells. However, it is also true that the ribosides themselves are a form of conjugation. All studies to date seem to indicate that the free-base has to be cleaved from the ribose too before the compound has any true activity. Plants carry out this reaction easily and rapidly, so ribosides appear to have activity on their own, but this is an artifact. Cells in culture require the cytokinin to be freethese sometimes lack the enzymes to cleave the ribose, so supplied ribosides are inactive and the free-base must be supplied in the medium. The native cytokinins also occur as modified bases in RNA and DNA strands. In fact cis-zeatin (a less active form) is found in many tRNA molecules in almost all living cells of all species! The extent to which the free cytokinin pool is altered by conjugation with other nucleotides or released from nucleotide polymers is not clear. CYTOKININS DEGRADATION: In addition to synthesis and conjugation, the pools of cytokinins can be altered by degradation. Below is how one natural cytokinin is made inactive: REFERENCES http://www.plant-hormones.info/cytokinins.htm Kieber JJ (March 2002). Tribute to Folke Skoog: Recent Advances in our Understanding of Cytokinin Biology. J. Plant Growth Regul. 21 (1): 1–2. doi:10.1007/s003440010059. PMID11981613. Campbell, Neil A.; Reece, Jane B.; Urry, Lisa Andrea.; Cain, Michael L.; Wasserman, Steven Alexander.; Minorsky, Peter V.; Jackson, Robert Bradley (2008). Biology (8th ed.). San Francisco: Pearson, Benjamin Cummings. pp.827–30. Chen CM, Ertl JR, Leisner SM, Chang CC (July 1985). GOVERNMENT COLLEGE UNIVERSITY,LAHOREPage 1

Florida International University Essay -- Environment, Waste, Green Pr

Florida International University (FIU) is not only a standard in quality education as it is also a innovator in best practices especially those that have to do with the environment. The University’s commitment to ensuring that its community contributes to the preservation and protection of the environment has led it to pioneer innovations in solid waste management, in particular, in recycling; because the University clearly understands that recycling is more beneficial compared to waste disposal, it has established its own reputation in the proper and efficient management of solid waste. Florida International University recognizes its contribution to the accumulation of waste considering that it produces about seven million pounds of waste each year (Figueroa, 2010). Unlike other universities that still remain adamant in investing on green practices, FIU has, â€Å"governed by the State of Florida under Florida Statute 403.714 and The Florida Solid Waste Management Act of 1988† (FIU, 2011), established its own internal ‘green machine’ or solid waste and environmental protection system via the FIU Custodial Services Office. The university’s efforts in ensuring efficient solid waste management even â€Å"far exceeds the minimum standards† (FIU, 2011) required by law. What makes the solid waste management efforts of the university one-of-a kind is the establishment of a â€Å"single stream recycling program which means that all recyclable items can be placed into one bin and do not have to be sorted† (FIU, 2011). Nevertheles s, despite the use of single recycle bins, proper labeling has to accompany each bin to ensure that only materials that are recyclable are placed in the bins. There are also different sized of bins which are placed stra... ...g, however, costs only $35 per ton. The school also makes $10 for every ton of paper it recycles† (Figueroa, 2009). This means that apart from saving the environment, the university also earns and is able to cut down on expenses for certain things because of the use of recycled materials. Of course, the most important aspect of recycling in the university is awareness and with students who are currently seriously considering the way they manage their solid waste, the campaign and program becomes even more successful. To do this, the university has also joined the national FIU Recyclemania for two years now and had placed exceptionally well (FIU, 2011). It is very important in any environmental effort that the stakeholders are made aware of the benefits of the efforts so that the program earns propriety and becomes a culture instead of just an obligation.